• 목록
• 답변
• 글쓰기
• 게시판 리스트 옵션
  • 수정
  • 삭제

Are recognized as unchanged, and the value of log(P/C) or log(O/C) is 0. e, - means that the ratio of P/C or O/C lower than 0.8 recognized as decreased expression, and the log values are under -0.097.p-values than the annotation clusters of brain proteins from rats fed perilla-diets. This suggests that the annotation clusters of the differentially expressed proteins in the hippocampus are not simple representations of the major protein groups in the brain.Western blot analysisTo verify the results of differential protein expressions in the brain upon perilla-diet intake, Western blotting analyses using antibodies against the four selected proteins were performed. Selection of protein was carried out according to the availability and specificity of the antibodies, reproducibility and fold differences of the proteomic results, and involvement of the proteins in synaptic plasticity.Since AMPA receptor subunits are essential for hippocampal synaptic plasticity, spatial learning and memory, as well as long term potentiation induction and maintenance [15-17], an antibody against AMPA receptor was used to investigate improved spatial learning and memory in rats supplemented with perilla-diets. After 3 weeks of perilla-diet intake, there was a significant increase in AMPA PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25751659 receptor expression, a positive control for learning and memory improvement, in the hippocampus (Figure 3). AMPA receptor protein was also differentially expressed in the hippocampus of rats supplemented with perilla-diets for 3 months. -synuclein protein showed a good correlation with the proteomic data from Western blotting analysis. Protein expression in the hippocampus significantly increased upon bothLee et al. Proteome Science 2012, 10:72 http://www.proteomesci.com/content/10/1/Page 5 ofTable 2 Differentially expressed proteins in Gemcitabine (hydrochloride) the hippocampus of rats fed perilla-diets for 3 monthsAccession number P62260 P63018 P37377 P07323 P10719 P23565 P06761 P12839 P85969 P63018 P46462 P70566 P63004 O88989 P61158 P04764 Q05982 P11598 P02770 P27605 Q9JJM9 P47942 P53534 P05708 P21575 Q9QUL6 MOWSE score 2.17E+12 3.93E+06 2069 1.38E+14 1.14E+13 1.30E+13 1.82E+16 4.40E+08 1.84E+13 2.91E+07 8.33E+16 3.88E+06 8881 743497 1.10E+07 2.91E+09 4.87E+07 8.32E+10 1.07E+10 101710 4.31E+09 8.82E+08 1.34E+11 1.79E+14 2.18E+18 1.49E+10 Coverage ( ) 67.1 19.8 37.9 55.1 33.3 42.6 39.1 22.5 59.9 22 40.3 31.9 21 27.2 24.4 39.6 65.1 33.9 30.3 34.9 36.6 28.8 27.1 24.8 37.8 20.6 Identified protein 14-3-3 protein epsilon Heat shock cognate 71 kDa protein Alpha-synuclein Gamma-enolase ATP synthase subunit beta, mitochondrial Alpha-internexin 78 kDa glucose-regulated protein Neurofilament medium polypeptide Beta-soluble NSF attachment protein Heat shock cognate 71 kDa protein Transitional endoplasmic reticulum ATPase Tropomodulin-2 Platelet-activating factor acetylhydrolase IB subunit beta Malate dehydrogenase, cytoplasmic Actin-related protein 3 Alpha-enolase Nucleoside diphosphate kinase A Protein disulfide-isomerase A3 Serum albumin Hypoxanthine-guanine phosphoribosyltransferase Septin-5 Dihydropyrimidinase-related protein 2 Glycogen phosphorylase, brain form Hexokinase-1 Dynamin-1 Vesicle-fusing ATPase log(P/C) + + + + + + + 0 0 + 0 0 0 log(O/C) + + + + + + 0 + 0 0 + 0 + + + + + + +perilla- and perilla oil-diet supplementation for 3 weeks or 3 months. Neurofilament was down-regulated in both the perilla-diet and perilla oil-diet groups after 3 weeks, but it was up-regulated in the 3 month perilla oil-di.